Composite

Part:BBa_K4463007:Design

Designed by: Qiaowa Gong   Group: iGEM22_XJTLU-CHINA   (2022-09-18)


SUMO-CsgA-ShMT3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The attachment of ShMT to CsgA, which is the major curli subunit protein of E.coli, allows ShMT3 to form MT proteins in the outer membrane of e.coli, thus achieving the adsorption of metal ions in waste water. SUMO tag was added to improve the expression and solubility of CsgA-ShMT recombinant protein.



Source

SUMO is cloned from PET-28a plasmid, csgA is from E.coli and ShMT3 is from BBa_K4463005.

References