Composite
Part:BBa_K4463007:Design
Designed by: Qiaowa Gong Group: iGEM22_XJTLU-CHINA (2022-09-18)
SUMO-CsgA-ShMT3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The attachment of ShMT to CsgA, which is the major curli subunit protein of E.coli, allows ShMT3 to form MT proteins in the outer membrane of e.coli, thus achieving the adsorption of metal ions in waste water. SUMO tag was added to improve the expression and solubility of CsgA-ShMT recombinant protein.
Source
SUMO is cloned from PET-28a plasmid, csgA is from E.coli and ShMT3 is from BBa_K4463005.